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Speaker: Fang Huang, Assisstant Professor, Purdue University Location: LD 010

Revealing Subcellular Structures with Live-cell and 3D Fluorescence Nanoscopy

We are currently in an exciting era of biology where the inner-workings of cells can be explored by rapidly developing imaging methods. Labeling specificity and live cell compatibility make fluorescence microscopy an important tool in biomedical research. Its resolution, however, is limited by diffraction to ~250 nm, preventing us from resolving detailed structures within the cell. The recent advent of single molecule switching nanoscopy methods (SMSN, also known as PALM/STORM), overcomes this fundamental limit by stochastically switching single fluorophores on and off so that their emission events can be localized with high precision resulting in a reconstructed image with down to ~25 nm lateral resolution. However, its application has been largely limited to fixed and flat samples due to the poor temporal resolution, inferior resolution in z, and rapidly deteriorating resolution in thick samples.

I will present some of our most recent developments which synergistically combine novel algorithm designs, newly available sensors and novel instrumentation technologies to allow SMSN imaging in living cells and in 3D. I will demonstrate the capabilities of these new super-resolution systems by revealing subcellular structures from a diverse set of biological model systems including virus, bacteria, yeast, and mammalian cells.


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Refreshments at 3:00 pm in the Physics Conference Room LD 154B

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